Since an American scientist, Karry Mullis, invented PCR in 1983, PCR technology has been widely used and continuous upgrading.
Karry Mullis's idea was to use Two primers, Nucleotides and DNA polymerase, to make unlimited copies of DNA with the principle
of the PCR cycle three steps: Denaturing, Cooling, and DNA Amplifying.
The First commercial Real-Time PCR Instrument was created in 1996. The instrument monitors fluorescence in the PCR tube as it is happening. Real-Time PCR was measured using two primers and a probe to start the reaction binding with DNA strands, and then Taq polymerase copies the DNA by adding up complementary nucleotides. Because the fluorescence dye was attached to the Primer during the reaction increasing the DNA copies, the emitted lights can be captured and displayed on the computer screen in Real-time as the PCR is cycling.
The Power of Real-time PCR, also known as quantitative PCR or qPCR, shows when the PCR is doubling each cycle without stopping the reaction. Real-time PCR is used in Disease diagnosis and Medical research. Since the end of 2019, Covid started outbreaking that PCR or Real-time PCR has generally been introducing to our lives. Most of people have had a swab or given a saliva sample for one of those tests. Therefore, Real-time PCR or qPCR has widely been used to detect viruses or bacteria from respiratory infections. 
Nanjing Superyears Gene Technology Company provides a real-time PCR machine, also known as quantitative PCR (qPCR), a sensitive instrument for specific detection that can help advance genetic research and variation analysis.
We created the innovative General 2215 RT-qPCR that adopts all-in-one with an industrial art design, combining the analytical instrument, large touch screen, and computer. General 2215 supports multiple PCR analysis and High-resolution Melt Curve(HRM) analysis, compatible with mainstream reagent kits. Therefore, it will be the best assistant and your preferred RT-qPCR.
Product Parameters
| Items | Content |
| Performance Parameters | Detection Range | 9 orders of magnitude |
| Sensitivity | Single Copy Detection |
| Reliability | 99.7% reliability of distinguishing between 5000 copies and 10000 copies |
| Run time | 40 mins |
| Basic Parameters | Sample Capacity | 96-well |
| Reaction Volume | 10-30μl |
| Applicable Consumables | 0.1ml,96-well plate,8-stripe tube, Single tube |
| Temp.Control Range | 4-100°C |
| Max.Temp. Rise and Fall speed | 5.5°C / sec |
| Temp.Accuracy | ±0.25 °C |
| Temp.Uniformity | ± 0.5 °C |
| Temp.Control Method | Peltier Semiconductor |
| Light source | Tungsten Halogen Lamp |
| Detector | CMOS Image Sensor, Accurate Imaging for 96-well plate simultaneously, no time difference |
| Excitation Light Range | 470-640nm |
| Detection | 520-682nm |
| Filter System | 5 sets of excitation filters, 5 sets of emission filters |
| Applicable Fluorescent Dyes | FAM™/SYBR® Green I, VIC®/JOE , NED™/TAMRA™/ Cy3® , ROX™ / Texas Red® and Cy5® etc. |
| Operating Environment | Power | 100-240VAC，50/60Hz； |
| Temperature | 10 to 35 °C,Max. temperature change no exceed than 15 °C within 24hrs |
| Relative Humidity | 20%~80% (non~condensing) |
| Dimensions | Size (L x W x H) | 400×500×585mm |
| Weight | ~35kg |
Note: The General 2215 real-time PCR instrument is compatible with Low profile 0.1mL PCR flat-top transparent cap reaction tubes/eight-striped tubes, 96-well PCR reaction plates with no Skirted or Half Skirted, do not use 0.2mL PCR reaction tubes and raised caps.
Accuracy: Optical formation of the 96-well plate image simultaneously, avoiding the mechanical repositioning that causes the error, the results with a high degree of accuracy
High-end:The Classic Upper Camera Design, Higher Signal to Noise Ratio (SNR) than the Fiber-conducted method, Lower Reagent-consuming
Simple Design:Integrated Design with embedded computer, Space-saving
Widely Used:5-Color Fluorescence, Support Customer Personalized Dye
Flexible:Flexible System- Optional Heading module to adaptive change in different reaction system（Optional 0.1/0.2ml,Default Setting is 0.1ml）
● Medical clinical ✓ Infectious disease diagnosis and efficacy evaluation
✓ Diagnosis of tumor diseases
✓ Prenatal and postnatal testing
✓ Genetic disease diagnosis
✓ Drug metabolism testing
✓ Animal disease detection
● Food Safety
● Agricultural Science
● Scientific research
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