Y62 Plex 8-color Fluorescent Kit
The Feature of Y62 Plex 8-color Fluorescent Detection Kit
Eight-Color Fluorescence Technology: This kit is based on the mature eight-color fluorescence technology, which can allocate more locus ithan the traditional five- color fluorescence.
FRET Fluorescence: Using FRET as primer, higher sensitivity and color balance, Used for detection of emergency scene cases.
Internal Positive Control (IPC): Innovative Internal Positive Control (IPC) is helpful to determine the quality of samples and the success of PCR.
Locus: Eight-color fluorescence , Included 59 Y chromosome STR、3 Y-chromosome indel，covered the latest requirement of Public Security for 20 core locus and 15 optimal locus selection of Y database, as well as the 27 alternative selections.
Location: Y chromosome STR database construction, paternal relationship identification, individual identification.
Performance: In line with the Operation Instruction for detection of Human Fluorescence-labeled Y-STR Compound Amplification Detection Reagent in Forensic Science issued by the Ministry of Public Security, a high-quality Y chromosome STR database can be completed in one reaction. It has the high resolution, sensitivity and anti-depressant ability.
Forensic Dna Identification Kits Display
Forensic Reagent Kits Dimension
| Component
| Content
| Description of Component
|
| Pre-reaction Kit | 2.5× PCR Mix | 1000µL x 1 | Buffer solution
|
5x Primers
| 500µL x 1 | Used to amplify the primer mixture
|
PCR Grade Water
| 1000µL x 1 | Nuclease-free deionized ultrapure water
|
Control DNA 9948 (0.5ng/µL)
| 20µL x 1 | Standard positive contrast
|
| Taq DNA Polymerase | 100µL x 1 | Hot-start DNA Polymerase
|
Post-reaction Kit
| Allelic Ladder
| 25µL x 1 | Contains amplified alleles |
Orange-640
| 120µL x 1 | Internal size standard
|
Matrix Kit
| 8 Dye Matrix
| 50µL x 1 | Used for spectral calibration
|
Reaction System and Procedures of Y62Plex STR Detection Kit
| Component | 25µL System (µL) | 10µL System (µL) |
2.5× PCR Mix
| 10.0 | 4.0 |
5x Primers
| 5.0 | 2.0 |
PCR Grade Water
| 8.0 | 2.6 |
Control DNA 9948 (0.5ng/µL)
| 1.0 | 1.0 |
Taq DNA Polymerase
| 1.0 | 0.4 |
| Total Volume | 25.0 | 10.0 |
The Advantages of Y62 Plex 8-color Fluorescent Kit
Y62 adopts the world's first 8-color fluorescence and fluorescence energy resonance transfer technology, including all 35 loci, 25 candidate loci and 2 Y chromosome Indel in the core and optimization of Y database construction standard.
The product will greatly improve the efficiency of database construction and reduce the cost of database construction. And it covers all the loci of mainstream companies in the market today.
High resolution: The correct typing of 62 loci was obtained by single tube reaction, and the detection resolution was higher than other similar products available on the market
High individual identification rate and non-paternity exclusion rate: it has a higher cumulative individual identification and non-paternity exclusion rate. When used alone or in combination with other commercial kits, it can provide rapid and high-resolution genetic testing products and technologies for criminal investigation, judicial identification and paternity identification of public security.
Suitable for degradation samples: more loci can be arranged in small fragments (within 300bp or 220bp), suitable for some degradation samples. Extraction free amplification: suitable for direct amplification of FTA® card, filter paper, gauze and other inspection materials.
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